mouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse MFG-E8 genomic DNA true Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://biochemi.ifrec.osaka-u.ac.jp/english/" target="_blank">Lab HP</A> 開発年：2004年開発者：長田重一先生・華山力成先生ら、機関名：大阪大学大学院生命機能研究科129Sv の ES 細胞を用いて作成した MFG-E8 遺伝子欠損マウスC57BL/6Nに対して6回の戻し交配を行った。 RBRC01726 Kyoto Univ. R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] <a href='https://brc.riken.jp/mus/pcr01726'>Genotyping protocol -PCR-</a> Immunology and Inflammation Research 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 304, 1147-1150 (2004).<br>公表を前提とした学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は寄託者と別途協議を行う。利用者が本件リソース寄託後2年以内に理研BRCより提供を受けて得た研究成果の発表は寄託者（開発者）との共著とする。 B6;129-Mfge8<tm1Osa>/OsaRbrc B6;129-Mfge8<tm1Osa>/OsaRbrc MFG-E8 遺伝子 (Gene ID = 17304)のエクソン4〜6をネオマイシン耐性遺伝子に置換したマウス 国立大学法人京都大学 長田　重一 開発年：2004年 開発者：長田重一先生・華山力成先生ら、 機関名：大阪大学大学院生命機能研究科 129Sv の ES 細胞を用いて作成した MFG-E8 遺伝子欠損マウス C57BL/6Nに対して6回の戻し交配を行った。 Backcross to C57BL/6(Heterozygote x C57BL/6NCrlj) Shigekazu NAGATA B (1-3 months) Developed by Drs. Rikinari Hanayama and Shigekazu Nagata at Osaka University in 2004. A neomycin cassette replaced exons 4-6 of MFGE-8 gene. The mutant mice were backcrossed to C57BL/6N for at least 9 times. 注意点など特になし B6;129-Mfge8<tm1Osa>. Apoptotic cells are rapidly uptaken by macrophages. This process is essential to prevent inflammation and the autoimmune response against intracellular antigens released from dying cells. Milk fat globule epidermal growth factor 8 (MFG-E8) is a protein that specifically binds to apoptotic cells and enhance the uptake of apoptotic cells by macrophages. The MFG-E8 deficient mice developed splenomegaly and suffered from glomerulonephritis as a result of autoantibody production. MFG-E8 is considered to have a critical role in removing apoptotic B cells in spleen and lymph nodes, and its deficiency can lead to autoimmune disease. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 304, 1147-1150 (2004).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.<br>The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.<br>For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author. B（1〜3か月） MFG-E8 KO; MFG-E8 Knockout mouse MFG-E8 KO; MFG-E8 Knockout mouse <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/nov_2006_mm" target="_blank">Mouse of the Month Nov 2006</A>